Nitrogen metabolism

The nitrogen metabolism pathway is a metabolic capability that is same in Toxoplasma gondii and Plasmodium falciparum. This shows the utilisation of nitrate and nitrite from host as source of nitrogen and the de novo synthesis of non essential amino acids glutamate and glutamine from it. The nitrate and nitrite transporters present in the genome are also given in the table below.

 

Protein EC Number Gene id Protein localisation Localisation data source
Glutamate dehydrogenase 1.4.1.2 TGME49_249390 Mitochondrion Previous publication
Glutamate dehydrogenase 1.4.1.4 TGME49_293180 Cytosol Previous publication
Nitrate reductase 1.7.1.3 TGME49_262910 Cytosol Previous publication
Nitrite reductase 1.7.1.4 TGME49_207620 Plasma membrane Previous publication
Glutamate-ammonia ligase 6.3.1.2 TGME49_273490 Cytosol Previous publication
Carbamoyl-phosphate synthase 6.3.4.16 TGME49_215260 Cytosol Previous publication
Nitrite transporter none TGME49_209800 Plasma membrane Previous publication; GO annotation
Nitrite transporter none TGME49_229170 Membrane GO annotation
Nitrite transporter none TGME49_292110 Membrane GO annotation
Nitrate transporter none TGME49_305770    

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
Nitrate Host Carbamoylphosphate Pyrimidine metabolism
Nitrite Host L-Glutamine Glutamate metabolism
2-oxoglutarate Pyruvate metabolism, Tricarboxylic acid (TCA) cycle L-Glutamate Glutamate metabolism