Nitrogen metabolism

Nitrogen metabolism pathway is a metabolic capability which is same in the Coccidians Neospora caninum and Toxoplasma gondii and in Plasmodium falciparum. This shows the utilisation of nitrate and nitrite from host as source of nitrogen and the de novo synthesis of non essential amino acids glutamate and glutamine from it. The nitrate and nitrite transporters present in the genome are also listed in the table below.

 

Enzyme EC Number Gene id
Glutamate dehydrogenase 1.4.1.2 NCLIV_065590
Glutamate dehydrogenase 1.4.1.4 NCLIV_000130
Nitrate reductase 1.7.1.3 NCLIV_025010
Nitrite reductase 1.7.1.4 NCLIV_002590
Glutamate-ammonia ligase 6.3.1.2 NCLIV_034160
Carbamoyl-phosphate synthase 6.3.4.16 NCLIV_052220
Nitrite transporter none NCLIV_004050
Nitrite transporter none NCLIV_012840
Nitrite transporter none NCLIV_030110
Nitrate transporter none NCLIV_070110

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
Nitrate Host Carbamoylphosphate Pyrimidine metabolism
Nitrite Host L-Glutamine Glutamate metabolism
2-oxoglutarate Pyruvate metabolism, Tricarboxylic acid (TCA) cycle L-Glutamate Glutamate metabolism