Mannose and fructose metabolism

The mannose and fructose metabolism pathway presented here is same as the pathway for Toxoplasma gondii (http://www.llamp.net/?q=Mannose%20and%20fructose%20metabolism). The genes for all the enzymes in this pathway are identified in Neospora caninum genome. This pathway utilises fructose, a hexose (may be from host) and fructose-6-phosphate, a glycolysis intermediate for the production of important nucleotide sugars such as GDP-D-mannose and GDP-L-fucose. These compounds are essential substrates for glycosylphosphatidylinositol (GPI) anchor biosynthesis and synthesis of the anchors for N-glycans (N-glycans biosynthesis).

 

Enzyme EC Number Gene id
GDP-L-fucose synthase 1.1.1.271 NCLIV_014250
Hexokinase 2.7.1.1 NCLIV_039820
Mannose-1-phosphate guanylyltransferase 2.7.7.13 NCLIV_028400
GDP-mannose 4,6-dehydratase 4.2.1.47 NCLIV_015780
Mannose-6-phosphate isomerase 5.3.1.8 NCLIV_026860
Phosphomannomutase 5.4.2.8 NCLIV_016330

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
beta-D-Fructose-6P Glycolysis GDP-D-Mannose N-glycan biosynthesis
beta-D-Fructose Starch and galactose metabolism, Host GDP-L-Fucose N-glycan biosynthesis
D-Mannose Host