Aminosugars metabolism

The metabolic pathway of aminosugars metabolism in Cryptosporidium species is same as the pathway in Toxoplasma gondii. Cryptosporidium muris possesses gene orthologs for all the enzymes present in T. gondii. The enzyme 2.3.1.4 missing in Plasmodium falciparum and T. gondii is also missing in C. muris. This pathway is important for the post-translational modification, N-linked glycosylation of proteins. N-linked glycosylation is the attachment of oligosaccharide chains to Asn in the tripeptide sequence Asn-X-Ser/Thr (X - any amino acid except Pro) of proteins and this facilitates the correct folding of some proteins.

 

Enzyme EC Number Gene id
Glucosamine-phosphate N-acetyltransferase 2.3.1.4 Missing in annotation
Dolichyl-diphosphooligosaccharide-protein glycotransferase 2.4.1.119 CMU_007850
Dolichyl-diphosphooligosaccharide-protein glycotransferase 2.4.1.119 CMU_013170
UDP-GlcNAc:dolichyl-pyrophosphoryl-GlcNAc GlcNAc transferase 2.4.1.141 CMU_005550
Glutamine-fructose-6-phosphate transaminase 2.6.1.16 CMU_019860
UDP-N-acetylglucosamine diphosphorylase 2.7.7.23 CMU_012960
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase 2.7.8.15 CMU_039560
Phosphoacetylglucosamine mutase 5.4.2.3 CMU_018450

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
D-Fructose-6P Glycolysis    
L-glutamine Glutamate metabolism L-glutamate Glutamate metabolism
UTP Pyrimidine metabolism    
Dolichyl phosphate N-glycan biosynthesis Dolichyl pyrophosphate N-glycan biosynthesis