Proline is a non essential amino acid which is de novo synthesised from glutamate (glutamate metabolism pathway) in Neospora caninum and Toxoplasma gondii. This pathway shows the enzymes which catalyse the conversion of proline into 4-hydroxy-L-proline and ornithine. The absence of the enzyme arginase (converts arginine into ornithine) in T. gondii and N. caninum has led to the shortening of arginine and proline metabolism pathway in MPMP to proline metabolism pathway for T. gondii and N. caninum here. Although the activities of enzymes catalysing conversion of arginine to carbamoyl phosphate and ornithine were experimentally demonstrated in T. gondii , the genes for those enzymes were not identified in either T. gondii or N. caninum genomes (refer to methionine metabolism for more details).
|Enzyme||EC Number||Gene id|
List of genes annotated as tRNA-Pro in N. caninum genome
Sources and fates of metabolites
|Substrate||Source pathways||Product||Fate pathways|
|2-oxoglutarate||Pyruvate metabolism, Tricarboxylic acid (TCA) cycle||Succinate||Tricarboxylic acid (TCA) cycle|