Aminosugars metabolism

The metabolic pathway of aminosugars metabolism in Neospora caninum and Toxoplasma gondii is same as the pathway in MPMP (http://sites.huji.ac.il/malaria/maps/aminosugmetpath.html) as T. gondii and N. caninum possess gene orthologs for all the enzymes present in Plasmodium falciparum. The enzyme 2.3.1.4 missing in P. falciparum is also missing in Coccidians, T. gondii and N. caninum. This pathway is important for the post-translational modification, N-linked glycosylation of proteins. N-linked glycosylation is the attachment of oligosaccharide chains to Asn in the tripeptide sequence Asn-X-Ser/Thr (X - any amino acid except Pro) of proteins and this facilitates the correct folding of some proteins.

 

Enzyme EC Number Gene id
Glucosamine-phosphate N-acetyltransferase 2.3.1.4 Missing in annotation
Dolichyl-diphosphooligosaccharide-protein glycotransferase 2.4.1.119 NCLIV_021120
Dolichyl-diphosphooligosaccharide-protein glycotransferase 2.4.1.119 NCLIV_031690
UDP-GlcNAc:dolichyl-pyrophosphoryl-GlcNAc GlcNAc transferase 2.4.1.141 NCLIV_037860
Glutamine-fructose-6-phosphate transaminase 2.6.1.16 NCLIV_031610
UDP-N-acetylglucosamine diphosphorylase 2.7.7.23 NCLIV_040400
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase 2.7.8.15 NCLIV_019070
Phosphoacetylglucosamine mutase 5.4.2.3 NCLIV_040500

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
D-Fructose-6P Glycolysis    
L-glutamine Glutamate metabolism L-glutamate Glutamate metabolism
UTP Pyrimidine metabolism    
Dolichyl phosphate N-glycan biosynthesis Dolichyl pyrophosphate N-glycan biosynthesis