Proline metabolism

Proline is a non essential amino acid which is de novo synthesised from glutamate (glutamate metabolism pathway) in Cryptosporidium species [1]. This pathway shows the enzymes which catalyse the conversion of proline into 4-hydroxy-L-proline and pyrroline-5-carboxylate. The enzyme ornithine aminotransferase present in Toxoplasma gondii is absent in Cryptosporidium species. In addition, arginase (converts arginine into ornithine) present in Plasmodium falciparum is absent in Coccidia including Cryptosporidia and has led to the shortening of arginine and proline metabolism pathway in MPMP to proline metabolism pathway here.

 

Enzyme EC Number Gene id
Proline hydroxylase 1.14.11.2 CMU_032060
Pyrroline-5-carboxylate reductase 1.5.1.2 CMU_016970
Pro-tRNA ligase 6.1.1.15 CMU_020360

 

List of genes annotated as tRNA-Pro in C. muris genome

 

CMU_004560 CMU_004570 CMU_016960

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
Proline Glutamate metabolism 4-hydroxy-L-proline ?
2-oxoglutarate Tricarboxylic acid (TCA) cycle Succinate Tricarboxylic acid (TCA) cycle