Proline metabolism

Proline is a non essential amino acid which is de novo synthesised from glutamate (glutamate metabolism pathway) in Cryptosporidium species [1]. This pathway shows the enzymes which catalyse the conversion of proline into 4-hydroxy-L-proline and pyrroline-5-carboxylate. The enzyme ornithine aminotransferase present in Toxoplasma gondii is absent in Cryptosporidium species. In addition, arginase (converts arginine into ornithine) present in Plasmodium falciparum is absent in Coccidia including Cryptosporidia and has led to the shortening of arginine and proline metabolism pathway in MPMP to proline metabolism pathway here.

 

Enzyme EC Number Gene id
Proline hydroxylase 1.14.11.2 Chro.30441
Pyrroline-5-carboxylate reductase 1.5.1.2 Chro.60426
Pro-tRNA ligase 6.1.1.15 Chro.60505

 

List of genes annotated as tRNA-Pro in C. hominis genome

 

Chro.trn007 Chro.trn008 Chro.trn019

 

Open in a new window

 

 

Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
Proline Glutamate metabolism 4-hydroxy-L-proline ?
2-oxoglutarate Host Succinate Host