Nitrogen metabolism

Nitrogen metabolism pathway is a metabolic capability which is same in Toxoplasma gondii and Plasmodium falciparum. This shows the utilisation of nitrate and nitrite from host as source of nitrogen and the de novo synthesis of non essential amino acids glutamate and glutamine from it. The nitrogen metabolism pathway of Piroplasma species possess the capability to utilise nitrites and nitrates to produce glutamate and carbamoyl phosphate. The de novo synthesis of glutamine is absent in these species

 

Enzyme EC Number Gene id
Glutamate dehydrogenase (NAD-specific) 1.4.1.2 BBOV_III009680
Glutamate dehydrogenase (NADP-specific) 1.4.1.4 BBOV_IV010390
Nitrate reductase 1.7.1.3 BBOV_II005800
Nitrite reductase 1.7.1.4 BBOV_III001680
Carbamoyl-phosphate synthase 6.3.4.16 BBOV_III003590
Nitrite transporter none BBOV_I000410

 

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Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
Nitrate Host Carbamoylphosphate Pyrimidine metabolism
Nitrite Host L-Glutamate Glutamate metabolism