Asparagine and aspartate metabolism
This metabolic pathway is same as the MPMP pathway of asparagine and aspartate metabolism for Plasmodium falciparum. The amino acids asparagine and aspartate are non-essential and de novo synthesised in both Toxoplasma gondii and P. falciparum [1]. The enzyme aspartate transaminase present in this pathway can catalyse reversible conversion of glutamate and oxaloacetate to aspartate and 2-oxoglutarate depending on the availability of amino acids and TCA cycle intermediates. The enzyme asparagine synthase (.6.3.5.4) catalyses conversion of aspartate to asparagine. The enzymes aspartate carbamoyltransferase and adenylosuccinate synthase catalyse synthesis of substrates for pyrimidine biosynthesis, N-carbamoyl-aspartate and adenylosuccinate respectively.
Enzyme | EC Number | Gene id | Protein localisation | Localisation data source |
---|---|---|---|---|
Aspartate carbamoyltransferase | 2.1.3.2 | TGME49_291640 | Nucleus | Previous publication |
Aspartate transaminase | 2.6.1.1 | TGME49_248600 | Cytosol | Previous publication |
Aspartate-tRNA ligase | 6.1.1.12 | TGME49_202530 | Cytosol | Previous publication |
Asparagine-tRNA ligase | 6.1.1.22 | TGME49_270510 | Cytosol | Previous publication |
Adenylosuccinate synthase | 6.3.4.4 | TGME49_279450 | Cytosol | Previous publication |
Asparagine synthase | 6.3.5.4 | TGME49_253430 | Extracellular? | Previous publication |
List of genes annotated as tRNA-Asp in the T. gondii genome
TGME49_018430 | TGME49_018440 | TGME49_050860 | TGME49_053505 |
TGME49_072580 | TGME49_100633 | TGME49_116460 |
List of genes annotated as tRNA-Asn in the T. gondii genome
TGME49_032460 | TGME49_039450 | TGME49_093750 | TGME49_100645 |
TGME49_113800 |
Sources and fates of metabolites
Substrate | Source pathways | Product | Fate pathways |
---|---|---|---|
L-Aspartate | Host | Oxaloacetate | Tricarboxylic acid (TCA) cycle |
L-Glutamine | Glutamate metabolism | L-Glutamate | Glutamate metabolism |
Carbamoylphosphate | Nitrogen metabolism | N-Carbamoyl-L-aspartate | Pyrimidine metabolism |
GTP, IMP | Purine metabolism | Adenylosuccinate | Purine metabolism |
2-oxoglutarate | Pyruvate metabolism, Tricarboxylic acid (TCA) cycle | L-Glutamate | Glutamate metabolism |
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