Aminosugars metabolism

The metabolic pathway of aminosugars metabolism in Toxoplasma gondii is same as the pathway in MPMP (http://sites.huji.ac.il/malaria/maps/aminosugmetpath.html) as T. gondii possesses gene orthologs for all the enzymes present in Plasmodium falciparum. The enzyme 2.3.1.4 missing in P. falciparum is also missing in Coccidians, T. gondii and Neospora caninum. This pathway is important for the post-translational modification, N-linked glycosylation of proteins. N-linked glycosylation is the attachment of oligosaccharide chains to Asn in the tripeptide sequence Asn-X-Ser/Thr (X - any amino acid except Pro) of proteins and this facilitates the correct folding of some proteins.

 

Enzyme EC Number Gene id Protein localisation Localisation data source
Glucosamine-phosphate N-acetyltransferase 2.3.1.4 Missing in annotation    
Dolichyl-diphosphooligosaccharide -protein glycotransferase 2.4.1.119 TGME49_203970 Endoplasmic reticulum membrane Go annotation
Dolichyl-diphosphooligosaccharide -protein glycotransferase 2.4.1.119 TGME49_231430 Plasma membrane Go annotation; Previous publication
UDP-GlcNAc:dolichyl-pyrophosphoryl-GlcNAc GlcNAc transferase 2.4.1.141 TGME49_268340    
Glutamine-fructose-6-phosphate transaminase 2.6.1.16 TGME49_231350 Apicoplast Previous publication
UDP-N-acetylglucosamine diphosphorylase 2.7.7.23 TGME49_264780    
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephospho transferase 2.7.8.15 TGME49_244520 Membrane Go annotation
Phosphoacetylglucosamine mutase 5.4.2.3 TGME49_264650 Plasma membrane Previous publication

 

Open in a new window

 

 

Sources and fates of metabolites

 

Substrate Source pathways Product Fate pathways
D-Fructose-6P Glycolysis    
L-glutamine Glutamate metabolism L-glutamate Glutamate metabolism
UTP Pyrimidine metabolism    
Dolichyl phosphate N-glycan biosynthesis Dolichyl pyrophosphate N-glycan biosynthesis